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Error bars on stacked bar ggplot2

Tags:

r

ggplot2

plyr

I'm struggling to put error bars into the correct place on a stacked bar. As I read on an earlier post I used ddply in order to stack the error bars. Then that changed the order of the stacking so I ordered the factor. Now it appears the error bars are correct on one set of bars but not the other. What I want is a graph that looks like that below, just with the standard error shown with error bars. I'm listing the dput of the original data and the ddply data as well as the data set.enter image description here

Suz2$org <- factor(Suz2$org, levels = c('fungi','bacteria'),ordered = TRUE)

library(plyr) 
plydat <- ddply(Suz2,.(org, group, time),transform,ybegin = copy - se,yend = copy + se) 

colvec <-c("blue", "orange")

ggplot(plydat, aes(time, copy)) + 
  geom_bar(aes(fill = factor(org)), stat="identity", width = 0.7) +
  scale_fill_manual(values = colvec) +
  facet_wrap(~group,nrow = 1)+
  geom_errorbar(aes(ymax=ybegin , ymin= yend ),width=.5) +
  theme(panel.background = element_rect(fill='white', colour='white'), 
        panel.grid = element_line(color = NA),
        panel.grid.minor = element_line(color = NA),
        panel.border = element_rect(fill = NA, color = "black"),
        axis.text.x  = element_text(size=10, colour="black", face = "bold"),  
        axis.title.x = element_text(vjust=0.1, face = "bold"),
        axis.text.y = element_text(size=12, colour="black"),
        axis.title.y = element_text(vjust=0.2, size = 12, face = "bold"))

dput(plydat)

structure(list(org = structure(c(1L, 1L, 1L, 1L, 1L, 1L, 1L, 
1L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L), .Label = c("fungi", "bacteria"
), class = c("ordered", "factor")), time = structure(c(1L, 1L, 
1L, 1L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L), .Label = c("0W", 
"6W"), class = "factor"), copy = c(97800000, 15500000, 40200000, 
10400000, 55100000, 14300000, 1.6e+07, 8640000, 2.98e+08, 77900000, 
2.33e+08, 2.2e+08, 3.37e+08, 88400000, 3.24e+08, 1.89e+08), group = structure(c(3L, 
4L, 1L, 2L, 3L, 4L, 1L, 2L, 3L, 4L, 1L, 2L, 3L, 4L, 1L, 2L), .Label = c("Native D0", 
"Native D707", "Notill D0", "Notill D707"), class = "factor"), 
    se = c(11100000, 2810000, 7110000, 2910000, 1.7e+07, 1500000, 
    1930000, 2980000, 43900000, 20100000, 56400000, 41200000, 
    75700000, 22500000, 57500000, 28100000), ybegin = c(86700000, 
    12690000, 33090000, 7490000, 38100000, 12800000, 14070000, 
    5660000, 254100000, 57800000, 176600000, 178800000, 261300000, 
    65900000, 266500000, 160900000), yend = c(108900000, 18310000, 
    47310000, 13310000, 72100000, 15800000, 17930000, 11620000, 
    341900000, 9.8e+07, 289400000, 261200000, 412700000, 110900000, 
    381500000, 217100000)), .Names = c("org", "time", "copy", 
"group", "se", "ybegin", "yend"), row.names = c(NA, -16L), class = "data.frame")

dput(Suz2)

structure(list(org = structure(c(1L, 1L, 1L, 1L, 1L, 1L, 1L, 
1L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L), .Label = c("fungi", "bacteria"
), class = c("ordered", "factor")), time = structure(c(1L, 1L, 
1L, 1L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L), .Label = c("0W", 
"6W"), class = "factor"), copy = c(97800000, 15500000, 40200000, 
10400000, 55100000, 14300000, 1.6e+07, 8640000, 2.98e+08, 77900000, 
2.33e+08, 2.2e+08, 3.37e+08, 88400000, 3.24e+08, 1.89e+08), group = structure(c(3L, 
4L, 1L, 2L, 3L, 4L, 1L, 2L, 3L, 4L, 1L, 2L, 3L, 4L, 1L, 2L), .Label = c("Native D0", 
"Native D707", "Notill D0", "Notill D707"), class = "factor"), 
    se = c(11100000, 2810000, 7110000, 2910000, 1.7e+07, 1500000, 
    1930000, 2980000, 43900000, 20100000, 56400000, 41200000, 
    75700000, 22500000, 57500000, 28100000)), .Names = c("org", 
"time", "copy", "group", "se"), row.names = c(NA, -16L), class = "data.frame")

Suz2

  org time     copy       group       se
1     fungi   0W 9.78e+07   Notill D0 11100000
2     fungi   0W 1.55e+07 Notill D707  2810000
3     fungi   0W 4.02e+07   Native D0  7110000
4     fungi   0W 1.04e+07 Native D707  2910000
5     fungi   6W 5.51e+07   Notill D0 17000000
6     fungi   6W 1.43e+07 Notill D707  1500000
7     fungi   6W 1.60e+07   Native D0  1930000
8     fungi   6W 8.64e+06 Native D707  2980000
9  bacteria   0W 2.98e+08   Notill D0 43900000
10 bacteria   0W 7.79e+07 Notill D707 20100000
11 bacteria   0W 2.33e+08   Native D0 56400000
12 bacteria   0W 2.20e+08 Native D707 41200000
13 bacteria   6W 3.37e+08   Notill D0 75700000
14 bacteria   6W 8.84e+07 Notill D707 22500000
15 bacteria   6W 3.24e+08   Native D0 57500000
16 bacteria   6W 1.89e+08 Native D707 28100000
like image 566
user2055130 Avatar asked May 19 '13 01:05

user2055130


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What is stacked bar graph?

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1 Answers

The values for both ybegin and yend, the range of the errorbar, are too low for the bacteria data. Since the bars for bacteria are on top of the fungi bars, the height of the fungi bars (plydat$copy[plydat$org == "fungi"]) has to be added to the errorbar values of the bacteria data.

plydat[plydat$org == "bacteria", ] 
   <- transform(plydat[plydat$org == "bacteria", ],
                ybegin = ybegin + plydat[plydat$org == "fungi", "copy"], 
                yend = yend + plydat[plydat$org == "fungi", "copy"])

enter image description here

like image 87
Sven Hohenstein Avatar answered Oct 12 '22 00:10

Sven Hohenstein