Jinja is a template engine for Python. It is similar to the Django template engine. A template engine or template processor is a library designed to combine templates with a data model to produce documents.
You want your if check to be:
{% if not loop.last %}
    ,
{% endif %}
Note that you can also shorten the code by using If Expression:
{{ ", " if not loop.last else "" }}
    you could also use the builtin "join" filter (http://jinja.pocoo.org/docs/templates/#join like this:
{{ users|join(', ') }}
    And using the joiner from https://jinja.palletsprojects.com/templates/#joiner
{% set comma = joiner(",") %}
{% for user in userlist %}
    {{ comma() }}<a href="/profile/{{ user }}/">{{ user }}</a>
{% endfor %}  
It's made for this exact purpose. Normally a join or a check of forloop.last would suffice for a single list, but for multiple groups of things it's useful.
A more complex example on why you would use it.
{% set pipe = joiner("|") %}
{% if categories %} {{ pipe() }}
    Categories: {{ categories|join(", ") }}
{% endif %}
{% if author %} {{ pipe() }}
    Author: {{ author() }}
{% endif %}
{% if can_edit %} {{ pipe() }}
    <a href="?action=edit">Edit</a>
{% endif %}
    
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