How to un-filter or clear filters in r dplyr?

Question

A diagram showing what I am trying to achieve:

Hello community. I am trying to mutate a new variable depending on the mean function of one specific group (filtered and grouped). As I try to create the new variable I ungroup the set to operate in all groups. I tried running this R code. However, the mutate function applies only to the filtered group and can't find an un-filter function. I dput() a sample of my data frame below (df01). Thank you very much in advance for all your comments and suggestions. Regards. M.

R code:

df01 %>% #summary table of the means to be used. 
  filter(GFPimg == "WT") %>% 
  group_by(Demineralization, Cond, Temp) %>%  
  summarise(Mean2 = mean(Mean)) %>% 
  arrange(desc(Demineralization))
  print() 

df01 %>%
  filter(GFPimg == "WT") %>% 
  group_by(Demineralization, Cond, Temp) %>% 
  mutate(mean2 = mean(Mean)) %>% 
  arrange(desc(Demineralization, Cond)) %>% 
  ungroup() %>% 
  group_by(Demineralization, Cond) %>%
  mutate(submean = Mean - mean2) %>% 
  print(n=200) 

Data frame sample df01:

df01 <- structure(list(ID = c(1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54), 
GFPimg = structure(c(1L, 1L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 2L, 2L, 2L, 2L, 3L, 3L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 3L, 3L, 3L, 3L, 3L, 3L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 2L, 2L, 2L, 2L, 3L, 3L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 3L, 3L, 3L, 3L, 3L, 3L, 1L, 1L), .Label = c("HT", "MT", "WT"), class = "factor"), 
Cond = structure(c(1L, 1L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 2L, 2L, 2L, 2L, 1L, 1L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 2L, 2L, 2L, 2L), .Label = c("EC", "EI"), class = "factor"), 
Temp = structure(c(2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L), .Label = c("37c", "RT"), class = "factor"), 
Side = structure(c(1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L, 1L, 2L), .Label = c("L", "R"), class = "factor"), 
Mean = c(62.435, 64.537, 102.447, 92.608, 103.277, 104.711, 67.017, 61.748, 68.921, 59.962, 63.368, 60.435, 69.54, 67.886, 51.71, 50.291, 50.881, 54.865, 80.538, 84.05, 92.223, 87.337, 90.444, 90.728, 29.951, 28.574, 30.896, 30.399, 29.773, 30.715, 31.498, 30.385, 99.004, 83.644, 95.962, 83.451, 22.649, 22.5, 53.066, 51.368, 55.459, 57.203, 54.444, 58.504, 76.518, 95.81, 23.43, 24.736, 28.86, 28.347, 28.386, 29.319, 58.017, 63.064, 80.293, 89.194, 70.52, 63.989, 71.436, 59.379, 75.986, 80.22, 71.583, 76.589, 77.138, 95.998, 77.193, 71.384, 75.614, 83.061, 73.062, 71.833, 71.83, 55.783, 77.376, 64, 96.14, 99.876, 40.972, 53.465, 36.25, 47.626, 40.619, 39.697, 34.34, 36.855, 77.131, 75.35, 67.014, 58.811, 39.237, 49.357, 74.333, 79.921, 62.631, 63.119, 60.207, 65.171, 77.563, 82.078, 39.115, 45.988, 42.65, 55.806, 33.534, 41.271, 62.359, 67.092), 
Demineralization = structure(c(2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L), .Label = c("After", "Before"), class = "factor")), class = "data.frame", row.names = c(NA, -108L))

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Answer 1

Since other users might have a similar question, I post the answer I arrived to.

Lessons learned:

1. The pipe operator (%>%) pass on a modified dataframe from the previous operation to the next one.
2. Subset() and filter() effectively produce a new dataframe that keeps only those rows that satisfy the condition, and drops all other rows.
3. There is no function to un-filter or clear filters or un-subset in dplyr. Instead, to keep all rows and perform a calculation on another specific subset, you can apply a conditional with ifelse().

Code that solved my problem:

df01 %>%
  group_by(Demineralization, Cond, Temp) %>% 
  mutate(Mean2 = mean(ifelse(GFPimg == 'WT', Mean, NA), na.rm=T)) %>% 
  ungroup() %>% 
  mutate(submean = Mean - Mean2) %>% 
  print(n=200)