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centerline of a polygonal blob (binary image)

I have a binary image of a worm (blob extraction which works well). I am interested in fitting a centerline on the blob (worm).

So far I came up with this: starting from a polygon (after outline extraction of blob in the image) I applied a voronoi computation and discarded all vertices which are outside of the polygon (blue) which gave me the black center line which I can further use to fit a smooth centerline.

However, this computation is not at all robust (due removing voronoi vertices not inside the polygon?) Does somebody know an algorithmic workflow to get the centerline of a polygon or the center line of a blob object (binary image). (skeletonization?, triangulation of polygon an using midpoints of inside edges)

Here is a demonstration:

enter image description here

like image 912
Gabriel Avatar asked Jun 14 '16 19:06

Gabriel


1 Answers

I have found a better solution:

  • skeltonize the binary blob
  • build the graph (without cycles) of the skeleton by tracing pixel lines and branch points
  • get all shortest paths from each end node to all other end nodes and take the longest one (approximately the start and end of the blob)
  • the resulting path from one end node (degree=1) to another end node is an approximation of the worm middle line.

If someone is interested in the solution, I may share a python notebook. end result: green= final midline graph, dark green=final midline

the graph of the skelton

Source Code:
The code to this question can be found here: https://github.com/gabyx/WormAnalysis (if you use it please star it)

like image 79
Gabriel Avatar answered Oct 04 '22 07:10

Gabriel