The Web Server Could Not Find the Requested Resource visual studio debugging

Question

Issue

I have a .net 4.0 Web Application running in visual studio 2012, every time I go to debug the site i'm receiving the following error:

The Web Server Could Not Find the Requested Resource

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What I've tried

I've followed microsoft's website with solutions: msdn

1. From a command prompt window, run the following command: systemroot\Microsoft.NET\Framework\ versionNumber \aspnet_regiis -i
2. I've also attempted to remove the .csproj.user files
3. Since we use Source Control i've deleted all items, the site and apppool and reconfigured from scratch.
4. AppPool is set to allow 32-bit applications

All to no avail...Anyone have a solution that might solve this visual studio debugging nightmare for me?