chrome.browserAction.onClicked.addListener(function(tab) {
  chrome.tabs.executeScript(
      null, {file : "app.js"});                                                                                                                                                                         
});
I am injecting a code like this to the extension on click. But I want to remove this injected code when the user clicks the extension Icon second time. How is it possible. The code Injects an html div with id "pluginHolder". I can manually remove it using the basic js code document.getElementById('pluginHolder').remove();. 
How to do this process dynamically?
Thanks in advance
I think the simplest solution something like
if(document.getElementById('pluginHolder')) {
    document.getElementById('pluginHolder').remove()
} else {
   var pluginHolder = document.createElement('div');
   document.body.appendChild(pluginHolder);
}
If you want to affect js code and not DOM.
you can use play with eventListener ,
 function logMouse() {
      console.log(e.x+':'+e.y);
}
// Initialize on first run to true else use old value NOT(!) ( last time injected script)
isEnable = isEnable ? !isEnable : !!isEnable;
if(isEnable) {
    window.addEventListener('mousemove', logMouse);
} else {
    window.removeEventListener('mousemove', logMouse);
} 
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