I have the following in my app.js file:  
var mysql = require('mysql');
var connection = mysql.createConnection({
    host: 'localhost',
    port: 3306,
    user: 'user',
    password: 'password',
    database: 'mydatabase'
});
connection.connect();
In routes/index.js, I currently have only the boilerplate code: 
var express = require('express');
var router = express.Router();
module.exports = router;
How do I make available the connection object from the app.js file in routes/index.js?
- the mysql connection pool is lazy, only creating and restoring connections as needed. with this keepalive, the pool is no longer lazy. once a connection is opened, the keepalive will keep it open. the pool no longer scales depending on traffic.
Nodejs MySQL Integration: Pool Connectionsvar pool = mysql. createPool({ connectionLimit: 7, host: 'localhost', user: 'root', password: '', database: 'todoapp' });
MySQL Driver for Node Projects Assuming that the server is running, you can communicate with it programmatically through a Node application, using a driver. For an application to have this ability, you need to install a MySQL Driver. A driver is available on npm as mysql !
I ended up splitting the database connection logic from the app.js file.  In a separate file called connection.js, I have the following: 
var mysql = require('mysql');
var connection = mysql.createConnection({
    host: 'localhost',
    port: 3306,
    user: 'user',
    password: 'password',
    database: 'mydatabase'
});
module.exports = connection;
Then in my route file, I add
var connection = require('../connection');
to the top of the file where all my other modules are brought in.  In my instance, the connection.js file is one level higher than my route file, hence the ../ in the require() function parameter. 
My preference is to do some simple dependency injection and pass the required resource into the router by wrapping the module in a function:
var express = require('express');
module.exports = function (connection) {
    var router = express.Router();
    //do stuff with the connection
    return router;
}
Then you just instantiate the router module in app.js as a function with the database connection as an argument:
app.use('/where/ever', require('./module-b')(connection)); 
Usually I wrap up the dependencies in an object:
app.use('/where/ever', require('./module-b')({db:connection})); 
This way you don't have to keep changing the function signature as dependencies are added. This gives you a super-lightweight inversion of control for your express applications.
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