Currently I am working on web based application. I want to know what are the key factors a designer should take care while designing scalable web based application ?
That's a fairly vague and broad question and something you could write books about. How far do you take it? At some point the performance of SQL JOINs breaks down and you have to implement some sharding/partitioning strategy. Is that the level you mean?
General principles are:
Having a good read of highscalability.com should give you some ideas. I highly recommend the Amazon articles.
Every application is different. You'll have to profile your application to see where you should concentrate your optimization efforts. Some web applications might require database access optimizations, while others have complicated business logic that cause the bottleneck.
Don't attempt to optimize random arbitrary parts of you application without first profiling. You might end up having to support complicated optimized code that doesn't actually make your application snappier.
I get the sense from the other answers here that there is a general confusion between scalability and performance. High performance means that the response is quick. High scalability means that you get a response no matter how many others are also using the site at the same time. There's a big difference.
In fact, you actually have to sacrifice a little performance just to get good scalability. A general pattern to scalability is distributed computing. Factoring functionality out into separate tiers of clustered servers (web, business rules, database) is the usual approach to scalability. That extra round trip will slow down page load a little bit.
Everyone always wants to focus on high scalability but also don't forget that, for software vendors who sell licenses to customers who self host the application, scaling down can be just as important as scaling up. An application that can run on a single server for ten users but can also be configured to run on a ten server web cluster, a three server middle tier, and a four server database cluster for 10,000 users would be a system well designed for scalability.
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