In my program, when I clicked the "question mark button" it shows another form as shown in the image below. But the button's border is shown, how can I make it transparent?

The only way to alter the border of a 'standard' button is to draw it yourself. This would also be the best way to overcome your problems with the Image. Or you can set the flat appearance as below...
Me.button1.FlatAppearance.BorderSize = 0
You can do this on the load event if you want.
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