Yes you can use required attribute in HTML5. But remember, first value should be empty.
<select required>
<option value="">Please select</option>
<option value="first">First</option>
</select>
Here you get the more example:
http://dev.w3.org/html5/spec-author-view/the-select-element.html#the-select-element
Yes it has a required attribute, you can use it as follows
<select required>
<option value="" disabled selected>Choose</option>
<option value="first Option">First Option</option>
<option value="Second Option">Second Option</option>
</select>
HTML Select required Attribute (W3C)
You can do this way to make it look better
<select required>
<option hidden="" disabled="disabled" selected="selected" value="">Select subject</option>
<option value="first Option">First Option</option>
<option value="Second Option">Second Option</option>
</select>
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