Can I run ASP.NET 2.0 and 3.5 code on the same website? ...or, do I need to separate them by applications, and/or servers?
.NET 3.5 is 2.0 with a few extra libraries. So the answer is yes you can run them on the same web site. In fact you cannot even set a web application to run under 3.5. It just runs under 2.0. You can check the ASP.NET tab in the properties of an IIS site to see that there isn't even an option to run your application under 3.5.
As long as your server is running 3.5, you can run both.
As far as IIS is concerned, 3.5 and 2.0 are the same. What you have to be careful about is not mixing 1.1 and 2.0 in the same app pool.
Yes you can without issue.
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