I am trying to deploy Python Flask application in the Azure web app. I had create web app(Flask) and published my code. After publish, I am getting below error from the site.
The page cannot be displayed because an internal server error has occurred.
When check the Log, i could see the below error.

But this was happening only in my subscription(free subscription got with MSDN). But working fine in the Organisation subscription.
The <fastCGI> settings must be in the applicationHost.config file (in the system.webServer section) of IIS. Just putting it into web.config does not work (confirmed by testing it on a local IIS, not in Azure). An example configuration may look like this:
<fastCgi>
  <application
    fullPath="D:\home\Python27\python.exe"
    arguments="D:\home\Python27\wfastcgi.py"
    maxInstances="16"
    idleTimeout="21600"
    instanceMaxRequests="10000000"
    signalBeforeTerminateSeconds="60"
    xdt:Transform="InsertIfMissing"
    xdt:Locator="Match(fullPath)">
    <environmentVariables>
      <environmentVariable name="PYTHONHOME" value="D:\home\Python27" />
    </environmentVariables>
  </application>
</fastCgi>
You may want to adjust this configuration.
This should solve it for a local IIS where you can edit applicationHost.config. I'm not sure about Azure, but maybe you can find some hints here: https://github.com/Azure/azure-python-siteextensions/issues/2.
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