I would like to align like this
label1 TextBox1
label2 TextBox2
label3 TextBox3
I set FlowDirection to TopDown
but how can I do to align label1 with TextBox1 horizontally ?
Yes, you can do it. You have to remove the anchors of each control. In a FlowLayoutPanel, no anchors means middle vertical alignment. I hope it helps.
Use RightToLeft instead of TopBottom in the FlowLayoutPanel and set FlowBreak=True to each TextBox.
To align vertically Labels and TextBoxes, set Anchor=None on all of them (as @Francisco says below)

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