I have a 25000x3-matrix, with each row containing a x-, a y- and a z-value. Now I wanted to do a graphical plot out of these. But for using for example surf(Z) I have to use a mxn-matrix as Z with m equal the size of x and n equal the size of y. How can I reshape the matrix I have to the needed mxn-matrix? The problem is that my x- and y-values are no ints, but floats, so I assume that I have to do a interpolation first. Is that true? My data plotted with plot3 looks like:

The fact that your x- and y- values are not integers is not a problem at all. The real question is: are your (x,y) points forming a grid, or not ?
If your points are forming a grid, then you have to reshape your columns to form m-by-n arrays. You may need to sort your data according to the first, then second column and then use the reshape function.
If your points are not forming a grid, then you will have to make an interpolation. By chance the scatterinterpolant class can nicely help you in doing so.
As you can see, the data you are providing is neither given in a gridded way, nor is the point cloud clean. You could however try to do the following:
trisurfHere is a MATLAB code that does this:
%// Generate some points P
[X,Y] = ndgrid(0:30);
P = [X(:), Y(:), X(:).^2+Y(:)];
%%// Here the actual computation starts
[~,I] = unique(P(:,1:2),'rows'); %// Remove points with duplicate (x,y)-coords
P = P(I,:);
T = delaunay(P(:,1),P(:,2)); %// Triangulate the 2D-projection
surface = triangulation(T, P(:,1), P(:,2), P(:,3)); %// Project back to 3D
trisurf(surface); %// Plot
You may want to remove stray points first, though.
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