f = plt.figure(figsize=(12,10))
ax1 = f.add_subplot(411)
ax2 = f.add_subplot(422)
ax3 = f.add_subplot(423)
ax4 = f.add_subplot(424)
ax5 = f.add_subplot(425)
ax6 = f.add_subplot(426)
ax7 = f.add_subplot(427)
ax8 = f.add_subplot(428)
I want to increase space between two rows: ax1 and ax2-ax3. Other spaces should remain the same. Using "f.subplots_adjust(hspace = 0.2, wspace= 0.25)" adjusts the spacing for all subplots. What can I do to increase hspace for the top-most subplot only?

import matplotlib.pyplot as plt
fig, axs = plt.subplot_mosaic([['top', 'top'],['left1', 'right1'], ['left2', 'right2']],
constrained_layout=True)
axs['top'].set_xlabel('Xlabel\n\n')
plt.show()
This will make all the y-axes the same size. If that is not important to you, then @r-beginners answer is helpful. Note that you need-not use subplot mosaic, though it is a useful new feature.

If you are not worried about the axes sizes matching, then a slightly better way than proposed above is to use the new subfigure functionality:
import matplotlib.pyplot as plt
fig = plt.figure(constrained_layout=True)
subfigs = fig.subfigures(2, 1, height_ratios=[1, 2], hspace=0.15)
# top
axtop = subfigs[0].subplots()
# 2x2 grid
axs = subfigs[1].subplots(2, 2)
plt.show()

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