500 Internal Server Error accessing Image in IIS7

Question

I have created an ASP.Net MVC4 site and deployed it to IIS7.

We noticed that the images in the css were not showing.

Trying to directly access the image file like this http://.com/CircuitSearch/Images/CircuitSearchBackgroundTrans.png returns the 500 error. Yes the filepath is correct.

Odd part if another site hosted on the same IIS works fine and can display images.

Answer 1

Change authentication for the site to Anonymous authentication/ application pool identity